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Description |
Unit |
Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-23-320475 |
NB300-136 |
200kDa Neurofilament Heavy Antibody (NAP4)//Avian, Human,/Mouse |
1/EA |
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문의 |
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Subject
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200kDa Neurofilament Heavy Antibody (NAP4) |
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Description
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/ Avian, Human,/Mouse |
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Clonality
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Mono |
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Company
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Novus biologicals |
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Application
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ELISA, IHC-P, Western Blot |
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Conjugation
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Immunogen
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Contents
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Description:Summary: | Species: | Av, Ma, Hu | | Applications: | IHC-P, WB, ELISA | | Clonality: | Monoclonal | | | Gene: | NEFH | | Purity: | Ascites | | Host: | Mouse | | | Specificity: | Specifically recognizes the phosphorylated variant of NF-H subunit (~180-220 kDa), showing some weaker reactivity with phosphorylated forms of NF-M. | | Publications: | Antibody has been mentioned in at least 1 publication. | Details: | Clone: | NAP4 | | Isotype: | IgG1 Kappa | | Immunogen: | Native pig spinal cord neurofilaments | | Localization: | Cytoplasmic | Gene Symbol: NEFH | Entrez: | 4744 (Human) | | Related Genes: | NEFM, NEFL, NFASC, SOD1, PRPH, GFAP, LDLR, FH, CFH, MAP2, MAPT, IGFALS, NES, REN, PRNP, AGT, MBP, UCHL1, METAP2, ACHE, HOXA9, MT1M, MAPK1, VIM, CLDN6, IL6, PSEN1, GPR158, PSEN2, RSPO4, PKP1, CDK5, ATP2A2, GAP-43, RANGAP1, MAPK9, CA3, SLC17A5, TAC1, LIF | Background: Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight neurofilament subunit and runs on SDS-PAGE gels in the range 180-220kDA with some variation in different species. This monoclonal recognizes specifically the phosphorylated form of NF-H which is normally found only in axons in the adult nervous system. Since closely related phosphorylated epitopes are also found on NF-M, this antibody shows some reactivity with phosphorylated forms of this protein also. Interestingly, in many damage and disease states the phosphorylated form of NF-H accumulate in the perikarya of neurons. The antibody can be used to identify axons in tissue sections and in tissue culture. It can also be used to study phosphorylated neurofilament accumulations seen in many neurological diseases and following neuronal injury. Species: | Reactivity: | Human NF-H and all other mammals tested to date. Also recognizes avian NF-H. | Applications: | Uses: | NB 300-136 can be used on cryostat sections and on formalin-fixed cells in tissue culture. | | Dilutions: | ELISA , Western Blot 1:5000, Immunohistochemistry-Paraffin 1:1,000 | Packaging: | Buffer: | 0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA | | Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. | | Preservative: | 5mM Sodium Azide | | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
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