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Description |
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Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-23-320477 |
NB500-416 |
200kDa Neurofilament Heavy Antibody (NF-01)//Bovine, Human/Mouse |
1/EA |
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Subject
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200kDa Neurofilament Heavy Antibody (NF-01) |
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Description
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/ Bovine, Human/Mouse |
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Clonality
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Mono |
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Company
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Novus biologicals |
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Application
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ELISA, ICC, IHC-P, IHC-P, Western Blot |
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Conjugation
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Immunogen
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Contents
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Description:Summary: | Species: | Rt, Po, Mu, Ma, Hu, Bv | | Applications: | IHC-Fr, IHC-P, ICC, WB, ELISA | | Clonality: | Monoclonal | | | Gene: | NEFH | | Purity: | protein A purified | | Host: | Mouse | | | Specificity: | This recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa) of various species. | | Publications: | Antibody has not yet been mentioned in a publication. | Details: | Clone: | NF-01 | | Isotype: | IgG1 | | Immunogen: | A pellet of pig brain cold-stable proteins after depolymerization of microtubules. | Gene Symbol: NEFH | Entrez: | 4744 (Human) | | | 380684 (Mouse) | | | 24587 (Rat) | | Related Genes: | NEFM, NEFL, NFASC, SOD1, PRPH, GFAP, LDLR, FH, CFH, MAP2, MAPT, IGFALS, NES, REN, PRNP, AGT, MBP, UCHL1, METAP2, ACHE, HOXA9, MT1M, MAPK1, VIM, CLDN6, IL6, PSEN1, GPR158, PSEN2, RSPO4, PKP1, CDK5, ATP2A2, GAP-43, RANGAP1, MAPK9, CA3, SLC17A5, TAC1, LIF | Background: Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. When visualised using an electron microscope, neurofilaments appear as 10nm diameter fibres of indeterminate length that generally have fine wispy protrusions from their sides. They are particularly abundant in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. Neurofilaments are composed of a mixture of subunits, which usually includes the three neurofilament triplet proteins neurofilament light (NFL), neurofilament medium (NFM) and neurofilament heavy (NFH). Neurofilaments may also include smaller amounts of peripherin, alpha internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable. Some studies have shown that levels of neurofilament heavy and neurofilament light are elevated in patients with Alzheimer's disease, frontotemporal lobe dementia, and vascular dementia. This antibody distinguishes three subdivisions of the macaque precentral motor cortex. The greater size of the left versus the right superior temporal lobe was found to be due to increased axonal myelination and not due to increased number of glial cells or SMI 32 enumerated neurons, suggesting that the specialization for language in the left temporal lobe is related to increased speed of signal transmission. In cultures of murine cortex, this antibody labels a neuronal population with enhanced vulnerability to kainate toxicity, most of which are GABAergic and reveal kainate activated Ca2+ uptake. Species: | Reactivity: | Cross-reacts with all Mammals, a wide range of other species, Human, Rat, Mouse, Cow and Pig. Not yet tested in other species. | Applications: | Uses: | ELISA: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IHC-P: Use at an assay dependant dilution. IHC-Fr: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. | | Dilutions: | ELISA , Western Blot , Immunocytochemistry , Immunohistochemistry-Paraffin , Immunohistochemistry-Frozen | Packaging: | Buffer: | Preservative: 15mM Sodium Azide Constituents: PBS. pH 7.4 | | Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. | | Preservative: | 15mM Sodium Azide | | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
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