| Synonyms | eIF4E-binding protein 1, EIF4EBP1, PHAS-1, FocusOn094 |
| Swiss Prot Num | Q13541 |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 45 (S-T-TP-P-G). |
| Product Type | Antibodies |
| Storage | Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Gingras AC, et al. (1998) Genes Dev 12(4): 502-513. 2. Brugarolas J, et al. (2004) Genes Dev 18(23): 2893-2904. 3. Kumar V, et al. (2000) EMBO J 19(5): 1087-1097. 4. Moody CA, et al. (2005) J Virol 79(9): 5499-5506. 5. Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95(4): 1432-1437. |
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| Format | State: Liquid purified IgG |
| ? | Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. |
| ? | Buffer System: PBS(without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol |
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| Specificity | 4E-BP1 antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 45. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. |
| Application | Western Blot: 1:500~1:1000. Immunohistochemistry: 1:50~1:100. |
| Concentration | 1.0mg/ml |
| Focuson | 094 Antibodies to eukaryotic Initiation Factors (eIF) |
