| Synonyms | 5-Hydroxytryptamine Receptor 1E, 5-HT-1E, Serotonin Receptor 1E, S31, 5-HT1E Receptor, FocusOn042 |
| Swiss Prot Num | P28566 |
| Immunogen | Synthetic peptide - KLH conjugated, 3rd cytoplasmic domain of human |
| Product Type | Antibodies |
| Storage | Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C to -70 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
|
| Format | State: Liquid Ig fraction |
| ? | Purification: Immunoaffinity Chromatography |
| ? | Buffer System: Phosphate buffered saline containing 0.09% sodium azide |
|
| Specificity | This antibody detects 5-HT1E Receptor (HTR1E), 3rd cytoplasmic domain. |
| Application | Immunohistochemistry on paraffin sections (16 - 24 ?g/ml). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100°C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature. |
| Background | 5HT1E, a Serotonin Receptor, is not well studied. It is classified as an 5-HT1-like receptor because of its ligand-binding and effector-coupling characteristics. The human 5-HT1E receptor has been studied in an African green monkey kidney cell line, in which it modulated cAMP levels upon binding serotonin. Lack of structural variants in the human gene indicate a highly conserved protein. |
| Concentration | 1.0 mg/ml |
| Focuson | 042 Antibodies to 5-hydroxytryptamine (Serotonin) Receptors |
