| Synonyms | DCP, DCP1, Angiotensin-converting enzyme |
| Swiss Prot Num | P12821 |
| Immunogen | Purified ACE from human lung and kidney. |
| Product Type | Antibodies |
| Recommended Isotype Control | SM10P (for use in human samples), AM03095PU-N |
| Storage | Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Do not freeze working dilutions Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | 1. Danilov, S.M. et al.: Structure-Function Analysis of Angiotensin I-converting Enzyme Using Monoclonal Antibodies J.Biol.Chem. 269(43): 26806-26814. (1994). 2. Franke, F.E. et al.: Angiotensin-I-Converting Enzyme in the Human Body (Abstract) XV European Congress of Pathology, 3-8 September 1995, Copenhagen-Denmark 3. Metzger, R. et al.: Lack of Angiotensin-Converting Enzyme (ACE) in Endothelial Cells of the Human Kidney. XV European Congress of Pathology, 3-8 September 1995, Copenhagen-Denmark |
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| Format | Reconstitution: Restore with 0.5 ml distilled water. |
| ? | State: Lyophilized purified Ig fraction. |
| ? | Purification: Protein A chromatography. |
| ? | Buffer System: PBS, pH 7.2 containig 10 mg/ml bovine serum albumin (BSA) as a stabilizer and 0.1% Kathon as a preservative. |
| Protocols | Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature 1. Deparaffinize and rehydrate tissue section 2. Place slide in a cuvette with 250ml 0.01M citrate buffer, pH 6.0 3. Heat slide in a microwave oven for 2 x 7min. at 700Watt 4. Leave slide in the buffer for 20min for cooling 5. Wash in distilled water 6. Block endogenous peroxidase 7. Wash in PBS 8. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 9. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 10. Wash in PBS 11. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 12. Wash in PBS 13. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 14. Wash in PBS 15. Counterstain with Mayer's hemalum For further information and details see technical information |
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| Specificity | Angiotensin converting Enzyme (ACE), denatured. The epitope recognized is localized on the NH2-terminal side of the molecule. Topographically the degree of expression correlates with the proportion of smooth muscle cells and elastic fibres of the vessel wall. ACE is predominantely expressed by small muscular arteries and by arterioles, whereas small and large veins do not usually express ACE. However, no ACE could be detected in the endothelial cells of kidney. Constitutively ACE is expressed on endothelial cells in different diseases like hypertension, myocardial infarction, sarcoidosis, diabetes. |
| Application | Suitable for Immunohistochemistry on Paraffin sections: 8 ?g/ml (1:50); microwave pretreatment for antigen retrieval is recommended. Suggested positive control: Human lung. |
| Background | Angiotensin converting enzyme is an unspecific endopeptidase which regulates blood pressure by generation of angiotensin II and degradation of bradykinin. |
| Concentration | 0.4 mg/ml |
