| Synonyms | DCP, DCP1, Angiotensin-converting enzyme |
| Swiss Prot Num | P12821 |
| Immunogen | Denatured human kidney CD143 (Angiotensin converting enzyme). Spleen cells from immunised Balb/c mice were fused with cells of the mouse X36 - Ag8 - 653 myeloma cell line. |
| Product Type | Antibodies |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Metzger, R. et al. (1999) Distribution of angiotensin -converting enzyme (CD143) in the normal human kidney and in non-neoplastic kidney diseases. Kidney International. 56: 1442 - 1454 2. Balyasnikova, I. V. et al. (2003) Monoclonal antibodies to denatured ACE (CD143) broad species specificity, reactivity on paraffin sections and detection of subtle conformational changes in the C-terminal domain of ACE. Tissue Antigens. 61 (1): 49- 62. |
|
| Format | State: Liquid Tissue Culture Supernatant containing 0.09% Sodium Azide |
|
| Specificity | This antibody recognises CD143, also known as angiotensin-converting enzyme (ACE). The antibody recognises denatured ACE, binding to an epitope in the C domain. |
| Application | Immunohistochemistry on paraffin sections: 1:10 - 1:100; This product requires antigen retrieval using heat treatment prior to staining of paraffin sections; Sodium citrate buffer pH 6.0 is recommended for this purpose. Western Blot: 1/50 - 1/100. ELISA. |
| Background | CD143 exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of arterioles and lung capillaries), epithelial cells (especially in proximal renal tubules and in the small intestine), by some neuronal cells and variably on some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa. Study of CD143 may be relevant to fertility (especially sperm - egg interactions), atherosclerosis and in drug targeting to the lung. |
