| Synonyms | DCP, DCP1, Angiotensin-converting enzyme |
| Swiss Prot Num | P12821 |
| Immunogen | Human lung CD143 (Angiotensin converting enzyme). Spleen cells from immunised Balb/c mice were fused with cells of the mouse X36 - Ag8 - 653 myeloma cell line. |
| Product Type | Antibodies |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Danilov, S. M. et al. (1994) Structure - function analysis of angiotensin - converting enzyme using monoclonal antibodies. Selective inhibition of N-domain active center. J. Biol. Chem. 269: 26806 - 26814. 2. Metzger, R. et al. (2000) CD143 in the development of atherosclerosis. Atherosclerosis 150: 21 - 31. |
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| Format | State: Liquid Tissue Culture Supernatant containing 0.09% Sodium Azide |
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| Specificity | This antibody recognises CD143, also known as angiotensin - converting enzyme (ACE). This antibody recognises active ACE binding to an N-terminal domain epitope different to that recognised by clone 9B9 (SM1658). |
| Application | Flow cytometry: Neat -1/10; use 10 ?l of the suggested working dilution to label 10e6 cells in 100 ?l. Immunoprecipitation. ELISA. Immunohistochemistry on frozen sections: Neat - 1/30. Recommended Positive Control: Kidney. |
| Background | CD143 exists in two forms, a 170kD somatic form and a 90kD germinal form. The somatic form is expressed by endothelial cells (especially those of arterioles and lung capillaries), epithelial cells (especially in proximal renal tubules and in the small intestine), by some neuronal cells and variably on some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa. |
