| Synonyms | DCP, DCP1, Angiotensin-converting enzyme |
| Swiss Prot Num | P12821 |
| Immunogen | Denatured Human kidney CD143 (angiotensin converting enzyme). Spleen cells from immunised Balb/c mice were fused with cells of the mouse X63 - Ag8 - 653 myeloma cell line. |
| Product Type | Antibodies |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Metzger, R. et al. (1999) Distribution of angiotensin-1 converting enzyme (CD143) in the normal human kidney and in, non-neoplastic kidney diseases. Kidney International 56: 1442-1454. 2. Balyasnikova, I.V. et al. (2003) Monoclonal antibodies to denatured ACE (CD143) broad species specificity, reactivity on paraffin sections and detection of subtle conformational changes in the C-terminal domain of ACE. Tissue Antigens. 61 (1): 49- 62. |
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| Format | State: Liquid Tissue Culture Supernatant containing 0.09% Sodium Azide |
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| Specificity | This antibody recognises CD143, also know as angiotensin - converting enzyme (ACE). This clone recognises denatured ACE, binding to an epitope in the C domain. |
| Application | Western Blot: 1/50 - 1/100. ELISA. Immunohistochemistry on paraffin sections: neat - 1/20; This product requires antigen retrieval using heat treatment methods prior to staining of paraffin sections. Recommended Positive Control Tissue: Kidney (lung in rat studies). |
| Background | CD143 exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa. Study of CD143 may be relevant to fertility (especially sperm-egg interactions), atherosclerosis and in drug targeting to the lung. |
