| Synonyms | DCP, DCP1, Angiotensin-converting enzyme |
| Swiss Prot Num | P12821 |
| Immunogen | Human lung CD143 (Angiotensin converting enzyme). Spleen cells from immunised Balb/c mice were fused with cells of the mouse X63 - Ag8 - 653 myeloma cell line. |
| Product Type | Antibodies |
| Recommended Isotype Control | SM10R (for use in human samples) |
| Storage | Prior to and following reconstitution store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Shelf life: one year from despatch. |
| References | 1. Danilov, S. M. et al. (1994) Structure - Function analysis of angiotensin - converting enzyme using monoclonal antibodies. Selective inhibition of N-domain active center. J. Biol. Chem. 269:26806 - 26814 2. Metzger, R. et al. (2000) CD143 in the development of atherosclerosis. Atherosclerosis. 150: 21 - 31 3. Danilov, S. et al. (1991) Lung is the target organ for a monoclonal antibody to angiotensin-converting enzyme. Lab. Invest. 64 (1): 118 - 124. |
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| Format | Reconstitution: Restore with 1 ml distilled water. |
| ? | State: Lyophilized purified IgG fraction. |
| ? | Label: R. Phycoerythrin (RPE) |
| ? | Purification: Affinity Chromatography on Protein G. |
| ? | Buffer System: Contains 0.09% Sodium Azide as preservative and 1% BSA as stabilizer. |
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| Specificity | This antibody recognises CD143, also know as Angiotensin-Converting Enzyme (ACE). This antibody recognises active ACE binding to an N-terminal domain epitope, different to that recognised by clone i2H5 (SM1656). |
| Application | Flow Cytometry: Use 10 µl of neat antibody to label 10e6 cells in 100 µl. |
| Background | CD143 exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa. |
| Concentration | 0.1 mg/ml |
