| Synonyms | KIAA0670 |
| Swiss Prot Num | Q9UKV3 |
| Immunogen | Synthetic peptide (TRTALHGVKWPQSNPK) correspoding to amino acids 1065 to 1080 of human AcinusL, 338 - 353 of human AcinusS or 307 - 322 of human AcinusS which are identical to mouse Acinus. Located near the C-terminus of the cleaved active peptide p17. |
| Product Type | Antibodies |
| Storage | The antibody can be shipped at ambient temperature. Store (in aliquots) at 2-8ºC. DO NOT FREEZE! Shelf life: one year from despatch. |
| References | 1. Condensed matter in cell death. Zamzami, N and Kroemer, G. , Nature, 1999 , 401, 127 - 8 2. Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation. Sahara S, et al., Nature, 1999, 401, 168 - 73 |
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| Format | State: Liquid purified Ig |
| ? | Purity: Purified |
| ? | Buffer System: Phosphate buffered saline solution containing 0.02% sodium azide as a preservative |
|
| Specificity | This antibody reacts to Acinus / ACIN1. |
| Application | Western Blot: 0.5 to 1 ?g/ml; detects a 220 kDa band in K562 cell lysate. |
| Background | Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE (1). A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusSu™ (2). |
| Concentration | 0.5 mg/ml |
