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Cat. No |
Model |
Description |
Unit |
Price (VATº°µµ) |
³³±â |
Àç°í |
DataSheet |
ÁÖ¹®/°ßÀû |
LCA-24-345344 |
BM5014 |
Adeno-Associated Virus 2 (AAV) - VP1/VP2// |
1/EA |
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Subject
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Adeno-Associated Virus 2 (AAV) - VP1/VP2 |
Description
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Clonality
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Mono |
Company
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Acris Antibodies GmbH |
Application
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Frozen sections, Immunofluorescence, Western blot / Immunoblot, Immunoprecipitation |
Conjugation
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Immunogen
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Contents
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Description: Manufacturer | Acris Antibodies GmbH | Quantity | 50 ug | Presentation | Purified | Applications | Frozen sections, Immunofluorescence, Western blot / Immunoblot, Immunoprecipitation | Isotype | IgG1 | Host | Mouse | Clone | A69 | PDF datasheet | view | Shipping to | Worldwide | Synonyms | AAV2 | Immunogen | Adeno-associated virus capsid proteins and virus particles. | Product Type | Antibodies | Recommended Isotype Control | AM03095PU-N | Storage | Store the antibody at 2-8°C after reconstitution. Avoid repeated freezing and thawing. Shelf life: one year from despatch. | References | 1. Wistuba A.,Weger S., Kern A., and Kleinschmidt J. (1995) Intermediates of adeno-associated virus type 2 assembly: Identification of soluble complexes containing Rep and Cap proteins. J. Virol. 69, 5311-5319. 2. Wistuba A., Kern A., Weger S., Grimm D., and Kleinschmidt J. (1997) Subcellular compartmentalization of adeno-associated virus type 2 assembly. J. Virol. 71, 1341-1352. 3. Wobus C.A., H?¨ùgle-D?¢Òrr B., Girod A., Petersen G., Hallek M. and Kleinschmidt J.A. (2000) Monoclonal antibodies against the adenoassociated virus type 2 (AAV-2) capsid: Epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virology 74, 9281-9293. | |
Format | Reconstitution: 1 ml distilled Water | ? | State: Lyophilized IgG1 | ? | Purification: Protein A affinity chromatography | ? | Buffer System: 0.5 % BSA and 0.09 % NaN3 in PBS | |
Specificity | A69 reacts with VP 1 and VP 2 of adeno-associated virus which are highly enriched in the nucleus. Epitope mapping experiments (Wobus et al., see references) identified aa169 to aa184 of VP2 and (with reduced intensity) aa123 to aa136 of VP1 capsid proteins as the specific binding region. Weak cross-reaction with serotypes 1, 3, and 6 | Application | Immunofluorescence microscopy. Immunoprecipitation. Immunohistochemistry on frozen sections: 1/10. Western Blot. Incubation time: 1h at RT. Dilution buffer: PBS or Tris-buffered saline. | |
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