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| Cat. No |
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Description |
Unit |
Price
(VAT별도) |
납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-24-345354 |
AM00315PU-N |
Adenovirus Type 2 + 5 E1A// |
1/EA |
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문의 |
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Subject
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Adenovirus Type 2 + 5 E1A |
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Description
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Clonality
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Mono |
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Company
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Acris Antibodies GmbH |
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Application
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Immunofluorescence, Paraffin Sections, Western blot / Immunoblot, Immunoprecipitation |
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Conjugation
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Immunogen
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Contents
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Description: | Manufacturer | Acris Antibodies GmbH | | Quantity | 0.1 mg | | Presentation | Purified | | Applications | Immunofluorescence, Paraffin Sections, Western blot / Immunoblot, Immunoprecipitation | | Isotype | IgG2a | | Host | Mouse | | Clone | M58+M73 | | PDF datasheet | view | | Shipping to | Not USA/Canada | | Synonyms | DM355P | | Immunogen | Adenovirus. | | Product Type | Antibodies | | Recommended Isotype Control | AM03096PU-N | | Storage | Store the antibody at 2 - 8 °C for one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. | | References | 1. Harlow E, et al. (1986)Mol Cell Biol, 6:1579-1589 2. Harlow E, et al. (1985) J Virol, 3:533-546. 3. Whyte P, et al. (1988) Nature, 334:124-129 4. Klein g, et al. (1987) Science 238:1539-1544 | |
| Format | State: Liquid ascites | | ? | Purification: Protein A chromatography | | ? | Buffer System: 10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide. | |
| Specificity | This antibody detects Adenovirus Type 2 + 5 E1A. Cellular Localization: Nuclear | | Application | Immunofluorescence. Immunoprecipitation (Native verified): Use Protein A; Ab 2 ?g/mg protein lysate. Western Blotting: Ab at 1?g/ml for 2 hrs at RT. Immunohistology (Formalin/paraffin): Use Ab at 1-2?g/ml for 30 minute at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Recommended positive control: 293 Cells or Adenovirus infected tissues. | | Background | The adenovirus early gene product E1A is a potent stimulator of cellular proliferation, which when overexpressed can overcome the growth-inhibitory effects of TGF-beta. The E1A region encodes a series of related proteins (35-46 kDa) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen. | | Concentration | 0.2 mg/ml | |
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