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Description |
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Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-24-345434 |
AP03032PU-N |
AKT2 / PKB beta//Human, Mouse, Rat, Bovine, Canine, Chicken, Guinea/Rabbit |
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Subject
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AKT2 / PKB beta |
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Description
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/ Human, Mouse, Rat, Bovine, Canine, Chicken, Guinea/Rabbit |
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Clonality
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Mono |
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Company
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Acris Antibodies GmbH |
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Application
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Enzyme Immunoassay, Western blot / Immunoblot |
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Conjugation
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Immunogen
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Contents
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Description: | Manufacturer | Acris Antibodies GmbH | | Quantity | 0.4 ml | | Reactivity | Human, Mouse, Rat, Bovine, Canine, Chicken, Guinea Pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus | | Presentation | Purified | | Applications | Enzyme Immunoassay, Western blot / Immunoblot | | Isotype | IgG | | Host | Rabbit | | PDF datasheet | view | | Shipping to | Worldwide | | Synonyms | RAC-PK-beta, Protein kinase Akt-2, Protein kinase B beta, Review 010 | | Swiss Prot Num | P31751 | | Immunogen | A five residue synthetic peptide based on the human Akt2, coupled to KLH | | Product Type | Antibodies | | Storage | Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. | | References | 1. Downward J (1998). Curr Opin Cell Biol. 10(2): 262-7. 2. Brazil DP, and Hemmings BA (2001). Biochem Sci. 26(11):657-64. 3. Nicholson KM, Anderson NG (2002). Cell Signal. 14(5): 381-95. 4. Hardie, G., Hanks, S. (1995). The Protein Kinase Facts Book, Protein-Serine Kinases Academic Press Limited, San Diego, CA 418pps. | |
| Format | State: Liquid immunoglobulin | | ? | Buffer System: 0.1 M Tris-acetate buffer, 0.09 % sodium azide | |
| Specificity | This antibody detects a ~65kDa protein, corresponding to the molecular mass of Akt2 PKB beta on SDSPAGE immunoblots. Does not react with PKB?± (Akt1) or PKB?³ (Akt3). | | Application | Western blot (3): 1:13000 dilution of SPC-148 was ufficient for detection of PKB?² in 20 ?¼g of HeLa cell lysate by ECL immunoblot analysis. ELISA. | | Background | kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growthfactor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3). | | Concentration | 0.25 mg/ml | | Review | r010 Antibody Panel to AKT Protein Family | |
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