Synonyms | ALB, BSA, HSA, Serum Albumin |
Swiss Prot Num | P02769 |
Immunogen | Albumin [Bovine Serum] |
Product Type | Antibodies |
Storage | Store vial at 2-8°C prior to restoration. Restore with 2.0 ml of deionized water (or equivalent). This product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration. |
References | (Conjugation) Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981. |
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Format | State: Lyophilised Ig fraction |
? | Label: Horseradish Peroxidase (HRP) |
? | Purification: Purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. |
? | Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Gentamicin Sulfate as preservative and 10 mg/ml Polyethlene Glycol (PEG-8000) as stabilizer |
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Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Albumin [Bovine Serum]. Cross reactivity against Albumin from other tissues and species may occur but have not been specifically determined. |
Application | Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. Recommended dilutions: ELISA 1:20,000 - 1:80,000 Western blot 1:2,000 - 1:10,000 Immunohistochemistry 1:500 - 1:2,500 |
Concentration | 10.0 mg/ml (by UV absorbance at 280 nm) |