|
| |
| Cat. No |
Model |
Description |
Unit |
Price
(VAT별도) |
납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-24-368788 |
AP16116PU-N |
ALMS1//Human, Mouse, Rat, Bovine, Canine/Goat |
1/EA |
|
|
|
 |
문의 |
|
|
|
|
|
|
|
Subject
|
ALMS1 |
|
Description
|
/ Human, Mouse, Rat, Bovine, Canine/Goat |
|
Clonality
|
Mono |
|
Company
|
Acris Antibodies GmbH |
|
Application
|
Paraffin Sections, Western blot / Immunoblot |
|
Conjugation
|
|
|
Immunogen
|
|
|
Contents
|
Description: | Manufacturer | Acris Antibodies GmbH | | Quantity | 0.1 mg | | Reactivity | Human, Mouse, Rat, Bovine, Canine | | Presentation | Aff - Purified | | Applications | Paraffin Sections, Western blot / Immunoblot | | Host | Goat | | PDF datasheet | view | | Shipping to | Worldwide | | Synonyms | Alstrom syndrome protein 1, KIAA0328 | | Swiss Prot Num | Q8TCU4 | | Immunogen | Peptide from C Terminus of the protein sequence according to NP_055935.4 | | Product Type | Antibodies | |
| Immunogen | Swiss Prot Num: Q8TCU4 | | ? | AA Sequence: C-RVTNQLLGRKVPWD | | ? | Genename: ALMS1 | | Storage | Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. | | References | Collin GB, Marshall JD, Ikeda A, So WV, Russell-Eggitt I, Maffei P, Beck S, Boerkoel CF, Sicolo N, Martin M, Nishina PM, Naggert JK. Mutations in ALMS1 cause obesity, type 2 diabetes and neurosensory degeneration in Alstrom syndrome. Nat Genet. 2002 May;31(1):74-8. PMID: 11941369 | |
| Format | State: Liquid Ig fraction | | ? | Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide | | ? | Buffer System: Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin | |
| Specificity | This antibody detects ALMS1 at C-term. | | Add. information | The C terminus of ALMS1 shares an 8 amino acid stretch with two other human proteins: hypothetical protein (XP_169104) and ERAL1 (NP_005693). However, this stretch is located internally in these two proteins and so will most likely not cross-react with this antibody. The N terminus was not chosen as the peptide is expected to cyclise during synthesis. | | Application | Peptide ELISA: Limit dilution 1:32000. Western blot: Preliminary experiments gave bands at approx 200kDa and 75kDa in lysates of Human T cell line Jurkat after 0.3 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 461kDa according to NP_055935.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: 1-3 µg/ml. In paraffin embedded Human Testis shows staining of cytoplasm in spermatocytes. | | Concentration | 0.5 mg/ml | | Species Reactivity | Tested: Human | | Expected from sequence similarity:Mouse, Rat, Dog, Cow | |
|
|
|
|
