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Cat. No |
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Description |
Unit |
Price (VATº°µµ) |
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DataSheet |
ÁÖ¹®/°ßÀû |
LCA-24-359327 |
AM05216PU-N |
P110 (mitochondrial membrane protein)//Human, Monkey/Mouse |
1/EA |
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Subject
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P110 (mitochondrial membrane protein) |
Description
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/ Human, Monkey/Mouse |
Clonality
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Mono |
Company
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Acris Antibodies GmbH |
Application
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Immunofluorescence, Western blot / Immunoblot |
Conjugation
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Immunogen
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Contents
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Description: Manufacturer | Acris Antibodies GmbH | Quantity | 100 ug | Reactivity | Human, Monkey | Presentation | Purified | Applications | Immunofluorescence, Western blot / Immunoblot | Isotype | IgG1 | Host | Mouse | Clone | 2G2 | PDF datasheet | view | Shipping to | Worldwide | Immunogen | Skeletal fracrtion of HeLa-S3 cells. | Product Type | Antibodies | Storage | Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch. | References | 1. Paulin-Levasseur M; Chen G; Lariviere C. The 2G2 antibody recognizes an acidic 110-kDa human mitochondrial protein, Histochem J, 30(9):617-25 1998. | |
Format | State: Liquid purified IgG fraction. | ? | Purity: Purified | ? | Purification: Purified form ascites. | ? | Buffer System: PBS with 0.08% Sodium Azide as presevative. | |
Specificity | Reacts with human and monkey mitochondria. It does not react with chicken, mouse or rat. | Application | Suitable for Western Blot (2-10 ug/ml) and Immunofluorescense. | Background | P110 is a human mitochondrial protein with a molecular weight of 110 kDa. Close examination of the staining pattern in HeLa and Fanconi's Anemia cells reveal differences in the morphology and organization of mitochondria in these two cell types. The epitope targeted may serve as a valuable marker in the investigation of relationships between mitochondria and other cellular structures in human cells. The mouse monoclonal P110 antibody labels mitochondria in human cells, as assessed by double staining with either Rhodamine 123 or a polyclonal antibody to mitochondrial matrix HSP-60 proteins. The P110 antigen has an approximate isoelectric point of 6.5 that copartitions with HSP-60 proteins during isolation of mitochondria from HeLa cells. The P110 staining pattern in HeLa and Fanconi's anaemia cells reveals differences in the morphology and organization of mitochondria in these two cell types. In HeLa cells, mitochondria appear as individual tubular compartments of variable length and are closely associated with vimentin filaments, particularly at the periphery of the nucleus. In Fanconi's anaemia cells, mitochondria have a filamentous shape and form an interconnected cytoplasmic reticulum running in parallel with both vimentin filaments and microtubules. After stabilization with aldehyde- or alcohol-based fixation protocols that optimize the preservation of cytoskeletal components, the epitope targeted by the 2G2 antibody may serve as a valuable marker in the investigation of relationships between mitochondria and other cellular structures in human cells. | Concentration | 1.0 mg/ml | |
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