Synonyms | DAP kinase 2 |
Swiss Prot Num | Q9UIK4 |
Immunogen | Synthetic peptide corresponding to C-terminal of human DAPK2 protein. |
Product Type | Antibodies |
Storage | Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch. |
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Format | State: Liquid purified Ig |
? | Buffer System: 10mM PBS, pH 7.4 with BSA and Sodium Azide |
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Specificity | This antibody reacts to DAPK2. |
Application | Immunohistochemistry on paraffin sections (1:50 for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Positive control: Breast carcinoma |
Background | Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFNγ induced apoptosis. A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1. Ectopicly expressed DAPK2 induced apoptosis in various types of cells. DAPK has high sequence homology to ZIP kinase and DRAK1/2, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. The messenger RNA of DAPK2 is expressed in multiple human tissues. |
Concentration | 0,2 mg/ml |