Synonyms | DNA Adenine Methylase |
Immunogen | Recombinant DNA Adenine Methylase protein. |
Product Type | Antibodies |
Storage | Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
References | 1. Pogolotti, AL Jr., et al. On the mechanism of DNA-adenine methylase. J Biol Chem, 1988, 263, 7461-7464. |
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Format | State: Liquid purified IgG fraction. |
? | Purity: Purified |
? | Buffer System: PBS containing 0.08% Sodium Azide as preservative. |
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Specificity | Species Bacteria. |
Application | Western blot (5-10 ?g/ml). |
Background | DNA adenine methylase (DAM) is an enzyme that methylates adenine residues in the 4-base pair DNA sequence, GATC. This methylation reaction has been shown to be crucial in a variety of cellular processes in bacteria. These include (i) the control of initiation of DNA replication (GATC occurs 11 times in the highly conserved bacterial origin of replication sequence); (ii) methyl-directed mismatch repair; (iii) control of transposition of certain transposable DNA elements; and (iv) control of gene expression. With regard to gene expression, the recent report by Heithoff et al. (Science; 284:967-970; 1999) showed that the deletion of the DAM gene rendered Salmonella typhimurium totally avirulent. |
Concentration | 1.0 mg/ml |