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Description |
Unit |
Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-23-348323 |
NB100-690 |
alpha Tubulin 4a Antibody (DM1A)//Bovine, Chicken/Mouse |
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문의 |
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Subject
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alpha Tubulin 4a Antibody (DM1A) |
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Description
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/ Bovine, Chicken/Mouse |
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Clonality
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Mono |
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Company
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Novus biologicals |
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Application
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IF, IHC-P, IP, Western Blot |
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Conjugation
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Immunogen
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Contents
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Description:Summary: | Species: | Xp, GP, Rt, Po, Mu, Hu, Ch, Bv | | Applications: | IHC-P, WB, IP, IF | | Clonality: | Monoclonal | | | Gene: | TUBA4A | | Purity: | protein G purified | | Host: | Mouse | | | Specificity: | This is specific to a 57 kDa protein identified as tubulin-alpha. It does not cross-react with tubulin-beta. | | Publications: | Antibody has been mentioned in at least 6 publications. | Details: | Clone: | DM1A | | Isotype: | IgG1 Kappa | | Immunogen: | Native chicken brain microtubules. | | Epitope: | aa 426-450 | Gene Symbol: TUBA4A | Entrez: | 7277 (Human) | | | 22143 (Mouse) | | | 500929 (Rat) | | Related Genes: | GAPDH, B2M, BDNF, TBP, RUNX1, VCL, PGK1, RABGAP1L, SERPINB4, SERPINB3, PPIA, PRDX1, S100A9, SOD2, HPRT1, EZR, TUBA1A, EPB41L3, TPM1, H2, PF4V1, E11, MASCOT, SLC24A3, STXBP1, EEF1A1, PSMD1, MYC, PRM1, TUFM, SGCA, RHCG, ARNTL, SEPT3, DNASE1, ITGB3, TUBA3C, CSTB, PHB, RPLP1 | Background: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle. Alpha tubulin is not suitable as loading control in adipose tissue as expression of tubulin in adipose tissue is very low ( Spiegelman and Farmer, Cell, 1982, 29(1):53-60) in cells undergoing adipose differentiation actin synthesis decreases by 90%. Excellent as a protein loading control antibody. Species: | Reactivity: | Cross-reacts with Chicken, Cow, Gerbil, Guinea pig, Human, Mouse, Pig, Rat, and Xenopus laevis. Not yet tested in other species. | Applications: | Uses: | Electron Microscopy: Use at an assay dependent dilution. Immunofluorescence: Use at an assay dependent dilution. Immunohistochemistry (paraffin-embedded sections): 1:25-1:50* (ABC method). Antigen retrieval is not essential but may optimise staining. Immunoprecipitation: 1:50* Western Blot: Use at a dilution of about 1/50 1/100*. Detects a band of approximately 57 kDa. This antibody is ideal for use as a Western blot loading control. Not tested in other applications. *Optimal dilutions/concentrations should be determined by the end user. | | Dilutions: | immunofluorescence , immunoprecipitation , Western Blot , Immunohistochemistry-Paraffin | | Concentrations: | 0.2 mg/ml | | Positive Controls: | 2 positive controls | Packaging: | Buffer: | PBS | | Storage: | Store at 4C. Do not freeze. | | Preservative: | Sodium Azide | | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
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