| Catalog Number | GTX80896 |
| Product Name | HA tag antibody |
| Product Description | Rabbit polyclonal to HA Tag |
| Synonyms | HA epitope tag |
| Background | Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Immunogen | Synthetic Peptide: YPYDVPDYA |
| Tested Applications | Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | For WB: Use at a dilution of 1:200. This antibody detects the presence of HA-tagged proteins. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Specificity | Detects HA tagged proteins |
| Target | HA tag |
| Form Supplied | Liquid |
| Purification | Affinity purified with antigen |
| Purification Note | From polyclonal serum |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.1 % sodium azide and 0.2% gelatin |
| Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Notes | Please inquire about the availability of the immunizing peptide. |
