Catalog Number | GTX82839 |
Product Name | HA antibody (Biotin) |
Product Description | Rabbit Polyclonal to Anti-HA EpitopeTag (Biotin) |
Background | Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the epitope tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered protein, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAGR, green fluorescent protein (GFP), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.FLAGR and anti-FLAGR are registered trademarks of Sigma-Aldrich Biotechnology Co. |
Clonality | Polyclonal |
Host | Rabbit |
Immunogen | GTX82839 immunizing peptide corresponds to the HA tag. Y P Y D V P D Y A |
Tested Applications | Western blot. The usefulness of this product in other applications has not been determined. |
Application Note | For WB: Use at a dilution of 1:500. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. |
Specificity | GTX82839 detects HA tagged proteins. This antibody is specific for the HA tag and does not detect other proteins. |
Target | HA |
Conjugation | Biotin |
Form Supplied | Liquid |
Storage Buffer | Phosphate-buffered saline containing 1 mg/ml BSA and 0.05% sodium azide |
Storage Instruction | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20¨¬C or below. Avoid freeze-thaw cycles. |