Synonyms | Tag from influenza hemagglutinin protein |
Immunogen | KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen. |
Product Type | Antibodies |
Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
References | 1. Marco Scarselli, et al. J. Biol. Chem., Feb 2009; 284: 3577-3585. (Applications: IF) 2. Yidan Lin, et al. Mol. Cancer Ther., Nov 2008; 7: 3556 - 3565. (Applications: WB) 3. Wang, Shucai, et al. Development 2007 Nov 01;134(21):3873-3882. (Applications: ChIP) 4. Zemskov, Evgeny A., et al. J. Cell Sci. 2007 Sep 15;120(18):3188-3199. (Applications: IF, WB) 5. Liu, Xiangguo, et al. Cancer Res. 2006 Dec 01;66(23):11115-11119. (Applications: WB). 6. Kolodziej, PA and Young RA. (1991) Methods Enzymol., 194:508-19. 7. Sells MA and Chernoff J. (1995) Gene, 152:187-9. |
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Format | State: Liquid purified Ig fraction. |
? | Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
? | Buffer System: PBS containing 0.09% (W/V) Sodium Azide as preservative. |
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Specificity | This antibody is specific to HA Tag. |
Application | ELISA: 1/5,000. Western Blot. Immunofluorescence. (See Ref. 1, 4.) ChIP. (See Ref. 3) |
Background | Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. This polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells. |
Caution | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Concentration | 0.25 mg/ml |