| Synonyms | E. coli SLT-1 alpha and beta |
| Immunogen | SLT Toxin from E. coli O157:H7 |
| Product Type | Antibodies |
| Recommended Isotype Control | AM03095PU-N |
| Storage | Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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| Format | Reconstitution: Restore with 1 ml distilled water |
| ? | State: Lyophilized purified IgG fraction. |
| ? | Buffer System: 10 mM Phosphate, pH 7.4 containing 0.9% Sodium chloride. |
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| Specificity | This antibody reacts with alpha, alpha-1 and beta subunits of SLT-1 (STX-1). Also reacts with alpha and alpha-1 subunits of SLT-2 (STX-2). |
| Application | Toxin Neutralization: Neutralized Vero Cell toxicity of E. coli O157:H7 extracts when used at a 10ug/ml concentration. Immunoblot: Detected SLT 1 when used at 1?g/ml concentration. ELISA: Used as capture antibody at 20 ?g/ml concentration (100?l); Immobilizes SLT 1 for detection by an HRP conjugate. |
| Background | Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins. |
| Concentration | 1.0 mg/ml (prior to lyophilization) |
