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Cat. No |
Model |
Description |
Unit |
Price (VATº°µµ) |
³³±â |
Àç°í |
DataSheet |
ÁÖ¹®/°ßÀû |
LCA-23-354814 |
NB100-92319 |
B Raf Antibody//Human, Mouse/Rabbit |
1/EA |
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¹®ÀÇ |
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Subject
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B Raf Antibody |
Description
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/ Human, Mouse/Rabbit |
Clonality
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Poly |
Company
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Novus biologicals |
Application
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ELISA, IHC-P, Western Blot |
Conjugation
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Immunogen
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Contents
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Description:Summary: Species: | Mu, Hu | Applications: | IHC, WB, ELISA | Clonality: | Polyclonal | | Gene: | BRAF | Purity: | Immunogen affinity purified | Host: | Rabbit | | Specificity: | Raf-B (Y198) detects endogenous levels of Raf-B (Y198) protein. | Details: Gene Symbol: BRAF Entrez: | 673 (Human) | Related Genes: | RAF1, MAPK1, ZHX2, EPHB2, KRAS, CDKN2A, HRAS, MAPK3, TP53, EGFR, NRAS, MAP2K1, MLH1, SNRPE, PIK3CG, PIK3CA, AKT1, RET, MAP2K2, PTEN, CTNNB1, MAPK12, ARAF, RAP1A, CDKN1A, MSH2, TERF2IP, APC, EGF, CCND1, ERBB2, PROC, CDK4, PIK3CB, PIK3CD, RASSF1, MGMT, TCEAL1, D4S234E, PKA | Background: Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases,ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted with highest levels in the cerebrum and testis. Species: Reactivity: | This antibody is reactive against Human, Mouse,. | Applications: Uses: | Western blot analysis of Raf-B (Y198) Antibody in extracts from HeLa cells. Immunohistochemistry analysis of Raf-B (Y198) Antibody in paraffin-embedded human brain tissue. | Dilutions: | ELISA 1:20000-1:40000, Western Blot 1:500-1:1000, immunohistochemistry 1:50-1:200, | Concentrations: | 1.0 mg/ml | Packaging: Buffer: | Phosphate buffered saline, pH 7.2. | Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. | Preservative: | 15mM Sodium Azide | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
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