Catalog Number | GTX13625 |
Product Name | E. coli [1031] antibody |
Product Description | Mouse monoclonal [1031] to E.coli |
Synonyms | Escherichia coli |
Background | Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins. |
Clonality | Monoclonal |
Clone No | 1031 |
Host | Mouse |
Isotype | IgG3 |
Immunogen | Tissue/cell preparation - boiled extract of O157 E. coli strain |
Tested Applications | ELISA, Immunofluorescence, Immunohistochemistry. The usefulness of this product in other applications has not been determined. |
Application Note | ELISA: Use at a dilution of 1/20 - 1/200. Functions as a capture antibody, detector antibody and pairs with itself. IF: Use at a dilution of 1/10 - 1/50. IHC: Use at a dilution of 1/10 - 1/50. Blotting: Use at a dilution of 1/10 - 1/50. GTX13625 immunoprecipitates LPS of O157:H7. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
Specificity | E. coli serotype O157 and immunoprecipitates LPS of O157:H7. |
Species Cross Reactivity | E. coli serotype O157 - Does not cross-react with E. coli serotypes: O111, O125, O20, O55, and K1 - Not yet tested in other species |
Target | E. coli |
Cellular Localization | Whole cell |
Form Supplied | Liquid |
Purification | Protein A purified |
Purification Note | From mouse ascitic fluid |
Storage Buffer | 0.01M Phosphate-buffered saline, pH 7.2, containing 0.1% Sodium azide |
Storage Instruction | Keep as concentrated solution. Aliquot and store at -20¨¬C or below. Avoid freeze-thaw cycles. |