|
|
Cat. No |
Model |
Description |
Unit |
Price (VATº°µµ) |
³³±â |
Àç°í |
DataSheet |
ÁÖ¹®/°ßÀû |
LCA-23-348613 |
NB600-472 |
E1 Ubiquitin Activating Enzyme Antibody//Human/Rabbit |
1/EA |
|
|
|
|
¹®ÀÇ |
|
|
|
|
|
|
Subject
|
E1 Ubiquitin Activating Enzyme Antibody |
Description
|
/ Human/Rabbit |
Clonality
|
Poly |
Company
|
Novus biologicals |
Application
|
ELISA, Western Blot |
Conjugation
|
|
Immunogen
|
|
Contents
|
Description:Summary: Species: | Hu | Applications: | WB, ELISA | Clonality: | Polyclonal | | Gene: | UBA1 | Purity: | IgG purified | Host: | Rabbit | | Details: Isotype: | IgG | Immunogen: | Recombinant full length UBE1 fusion protein (Human) | Gene Symbol: UBA1 Entrez: | 7317 (Human) | Related Genes: | BCHE, CELA3B, ENOPH1, UBA2, UCHL1, XIST, UBA3, UBE2D2, VCP, RPS4X, UBE2L6, TIMP1, ZFX, ARAF, UBE2D3, RNF123, TP53, UBE2K, NMNAT1, UBE2I, UBE2L3, SYN1, UBA7, UBE2D1, PCTK1, SRY, KDM5D, UBE2E3, PSMB8, PSMB10, PSMB9, UBA6, CDC34, USE1, A4GALT, UBE2R2, POLD1, B3GALNT1, CYBB, USP9X | Background: Ubiquitinylation, the covalent attachment of ubiquitin to protein, regulates a number of cellular processes including responses to stress. This process is responsible for selective targeting of proteins for degradation. The first step in ubiquitinylation is the activation of ubiquitin by ubiquitin activating enzyme E1 which is a rate-limiting enzyme in the conjugation process. The ubiquitin activating enzyme exists as two isoforms: E1a (117 kDa), localized predominantly in the nucleus, and E1b (110 kDa), localized in the cytoplasm. The first 11 amino acids are considered to be essential for phosphorylation and exclusive nuclear localization of the enzyme. Species: Reactivity: | Reacts with Human. Predicted to react with Mouse (95% identity with immunogen), Rat (95% identity with immunogen), Rabbit (96% identity with immunogen) and Dog (93% identity with immunogen) due to sequence homology. Not yet tested in other species. | Applications: Uses: | WB: Predicted MW 118 kDa. Optimal dilutions/concentrations should be determined by the end user. | Dilutions: | ELISA 1/2000 - 1/10000, Western Blot 1/1000 - 1/5000 | Concentrations: | 5.0 mg/ml | Positive Controls: | 1 positive controls | Packaging: Buffer: | 0.15M Sodium chloride, 0.02M Potassium phosphate. p | Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. | Preservative: | Sodium Azide | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
|
|
|
|