랩캠퍼스(Labcampus) : [항체] - F4/80 [CI:A3-1] antibody

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F4/80 [CI:A3-1] antibody

Brand : GeneTex

Cat. No	Model	Description	Unit	Price (VAT별도)	납기	재고	DataSheet	주문/견적
LCA-25-388467	GTX26640	F4/80 [CI:A3-1] antibody//Cross/Rat	1/EA					문의

Subject

F4/80 [CI:A3-1] antibody

Description

/ Cross/Rat

Clonality

Mono

Company

GeneTex

Application

FACS, IHC-Fr, IHC-P, IHC-R, IP, RIA, Western Blot

Conjugation

Immunogen

Contents

Image:GTX26640.gifPrint:Print

Catalog Number	GTX26640
Product Name	F4/80 [CI:A3-1] antibody
Product Description	Rat monoclonal [CI:A3-1] to F4 / 80
Synonyms	13733 \| Emr1 \| DD7A5-7 \| EGF-TM7 \| F4/80 \| Gpf480 \| Ly71 \| TM7LN3 \|
Background	The antibody binds to macrophages from different sites including the peritoneal cavity, lung, spleen and thymus, to blood monocytes and to macrophages derived from bone marrow precursors in culture. The antigen is not expressed by lymphocytes or polymorphonuclear cells.
Clonality	Monoclonal
Clone No	CI:A3-1
Host	Rat
Isotype	IgG2b
Immunogen	Thioglycollate stimulated peritoneal macrophages from C57/BL mice
Antigen Species	Mouse
Tested Applications	Flow cytometry/FACS, IHC (Frozen sections), IHC (Formalin-fixed paraffin-embedded sections), IHC (Resin sections), Immunoprecipitation, Radioimmunoassay, Western blot. The usefulness of this product in other applications has not been determined.
Application Note	Recommended dilutions: FACS: Use 10μl for 10^6 cells in 100μl. RIA: Use at an assay dependent dilution. WB: Use at a concentration of 10 μg/ml. Use under non reducing conditions. Detects a band of approximately 160 kDa (predicted molecular weight of precursor protein: 102 kDa; protein is heavily glycosylated). Block in 5% milk for 1 hour. IP: Use at an assay dependent dilution. IHC-R: Use at an assay dependent dilution. IHC-Fr: 1/1000. IHC-P: 1/50 - 1/100. This antigen is known to be sensitive to fixation: for less than 1 day fixation either no antigen retrieval, Trypsin or Proteinase K (6 min, RT) enzyme pretreatment is recommended. Citrate buffer pH6.0 is recommended for tissues fixed for more than 24 hours. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Specificity	This antibody recognises the mouse F4/80 antigen, a 160kD glycoprotein expressed by murine macrophages.
Species Cross Reactivity	Cross-reacts with Mouse. Not yet tested in other species.
Positive Controls	murine macrophages
Target	F4/80
Cellular Localization	Cell Membrane
Form Supplied	Liquid
Purification	IgG fraction
Storage Buffer	0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
Storage Instruction	Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.