Swiss Prot Num | Q9H9S0 |
Immunogen | This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human NANOG. |
Product Type | Antibodies |
Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
References | 1. Kochupurakkal,B.S., Biochem. Biophys. Res. Commun. 365 (4), 846-850 (2008). 2. Freberg,C.T., Mol. Biol. Cell 18 (5), 1543-1553 (2007). |
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Format | State: Liquid purified Ig fraction. |
? | Purification: Protein A Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
? | Buffer System: PBS with 0.09% (W/V) Sodium Azide as preservative. |
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Specificity | This antibody detects NANOG at Center. |
Add. information | Predicted Molecular weight: 34489 Da |
Application | ELISA: 1/1,000. Western blot: 1/50-1/100. Immunohistochemistry: 1/10-1/50. Immunofluorescence: 1/10 -1/50. |
Background | NANOG is a Ttranscription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation. |
Concentration | 0.25 mg/ml |