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Description |
Unit |
Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-24-366913 |
AP01515PU-N |
4E-BP1//Human, Mouse, Rat/Rabbit |
1/EA |
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Subject
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4E-BP1 |
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Description
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/ Human, Mouse, Rat/Rabbit |
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Clonality
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Mono |
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Company
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Acris Antibodies GmbH |
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Application
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Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot |
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Conjugation
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Immunogen
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Contents
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Description: | Manufacturer | Acris Antibodies GmbH | | Quantity | 100 ug | | Reactivity | Human, Mouse, Rat | | Presentation | Aff - Purified | | Applications | Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot | | Host | Rabbit | | PDF datasheet | view | | Shipping to | Worldwide | | Synonyms | eIF4E-binding protein 1, EIF4EBP1, PHAS-1, FocusOn094 | | Swiss Prot Num | Q13541 | | Product Type | Antibodies | | Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. | |
| Format | State: Liquid purified Ig | | ? | Purification: Affinity chromatography | | ? | Buffer System: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2. | |
| Specificity | p-4E-BP1 (pSer64) antibody detects endogenous levels of p-4E-BP1 protein. | | Application | ELISA: 1:1000~1:5000. WB: 1:500~1:1000. IHC: 1:50~1:200. | | Background | The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5' end of mRNA. The eIF4F subunit eIF4E interacts directly with the mRNA 5' cap structure. Assembly of the eIF4F complex i inhibited by a family of repressor polypeptides, the eIF4E-binding proteins (4E-BPs). 4E-BP1 (also known as PHAS-1) normally binds eIF4E, inhibiting cap-dependent translation. Hyper-phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. The PI3-kinase/Akt pathway and the FRAP/mTOR kinase regulate 4E-BP1. 4E-BP1 is phosphorylated in vivo on multiple residues and phosphorylation by FRAP/mTOR on Threonine 37 and Threonine 46 of human 4E-BP1 may prime it for subsequent phosphorylation at sites including Serine 65 and Threonine 70. The corresponding rat residues include Threonine 36, Threonine 45, Serine 64 and Threonine 69. In vitro, 4E-BP1 is also phosphorylated by ataxia telangiectasia (ATM) at human Serine 112 (rat Serine 111) in response to an increase in insulin levels. | | Concentration | 1,0 mg/ml | | Focuson | 094 Antibodies to eukaryotic Initiation Factors (eIF) | |
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