| Synonyms | eIF4E-binding protein 1, EIF4EBP1, PHAS-1, FocusOn094 |
| Swiss Prot Num | Q13541 |
| Immunogen | This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S100 of human EIF4EBP1. |
| Product Type | Antibodies |
| Storage | Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Fonseca,B.D., J. Biol. Chem. 282 (34), 24514-24524 (2007). 2. Armengol,G., Cancer Res. 67 (16), 7551-7555 (2007). |
|
| Format | State: Liquid Ig fraction |
| ? | Purification: Protein A column, followed by peptide affinity purification |
| ? | Buffer System: PBS with 0.09% (W/V) sodium azide |
|
| Specificity | This antibody detects EIF4EBP1. |
| Add. information | Molecular weight: 12449 Da |
| Application | ELISA 1:1,000. Western blot 1:50 - 1:100. Immunohistochemistry 1:10 - 1:50. |
| Background | EIF4EBP1 is one member of a family of translation repressor proteins. It directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. |
| Concentration | 0.25 mg/ml |
| Focuson | 094 Antibodies to eukaryotic Initiation Factors (eIF) |
