| Synonyms | eIF4E-binding protein 1, EIF4EBP1, PHAS-1, FocusOn094 |
| Swiss Prot Num | Q13541 |
| Immunogen | This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T36 of human EIF4EBP1. |
| Product Type | Antibodies |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Fonseca,B.D., J. Biol. Chem. 282 (34), 24514-24524 (2007) 2. Armengol,G., Cancer Res. 67 (16), 7551-7555 (2007) |
|
| Format | State: Liquid purified Ig fraction. |
| ? | Purification: Protein A Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| ? | Buffer System: PBS with 0.09% (W/V) Sodium Azide as preservative. |
|
| Specificity | This antibody detects 4E-BP1/EIF4EBP1 pThr36. Predicted to cross react with Mouse (100% Antigen Homology). |
| Add. information | Molecular weight: 12449 Da |
| Application | ELISA: 1/1,000. Dot Blot: 1/500. |
| Background | EIF4EBP1 is a member of a family of translation repressor proteins. This protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. It is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. |
| Caution | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Concentration | 0.25 mg/ml |
| Focuson | 094 Antibodies to eukaryotic Initiation Factors (eIF) |
