| Synonyms | eIF4E-binding protein 1, EIF4EBP1, PHAS-1, FocusOn094 |
| Swiss Prot Num | Q13541 |
| Immunogen | A 14 amino acid peptide located near human 4EBP1 carboxy-terminus |
| Product Type | Antibodies |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Hara, K. et al (1997) Regulation of eIF-4E BP1 phosphorylation by mTOR*. J. Biol. Chem. 272: 26457 - 26463. 2. Haghighat, A. et al. (1995) Repression of cap-dependent translation by 4E-binding protein 1: competition with p220 for binding to eukaryotic initiation factor-4E. EMBO J. 14: 5701 - 5709 |
|
| Format | State: Liquid purified IgG |
| ? | Purification: Affinity chromatography |
| ? | Buffer System: PBS containing 0.02% Sodium Azide |
|
| Specificity | This antibody recognises an epitope within the C-terminal region (CT) of eukaryotic translation initiation factor 4E binding protein 1 (4EBP1), a rapamycin-sensitive downstream target of TOR (Target of rapamycin), and regulatory factor of eukaryotic mRNA translation.The association between 4EPB1 and eukaryotic translation initiation factor 4E (eIF-4E) inhibits the binding of eIF-4E with p220, thereby preventing the formation of a functional eIF-4E complex, essential for mRNA translation. Phosphorylation of 4EBP1 following stimulation by mitogens such as insulin, results in the release of eIF-4E from 4EBP1, thereby enabling the translation complex to assemble. |
| Application | Western Blot: 2.5 - 5.0?g/ml; this antibody detects a band of approximately 20kDa in mouse 3T3 cell lysates (predicted Mwt. 13kDa). |
| Concentration | 1.0 mg/ml |
| Focuson | 094 Antibodies to eukaryotic Initiation Factors (eIF) |
