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Cat. No |
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Description |
Unit |
Price (VATº°µµ) |
³³±â |
Àç°í |
DataSheet |
ÁÖ¹®/°ßÀû |
LCA-24-372289 |
AP12602PU-N |
E2F1 Transcription Factor//Human/Rabbit |
1/EA |
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Subject
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E2F1 Transcription Factor |
Description
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/ Human/Rabbit |
Clonality
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Mono |
Company
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Acris Antibodies GmbH |
Application
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Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot |
Conjugation
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Immunogen
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Contents
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Description: Manufacturer | Acris Antibodies GmbH | Quantity | 0.1 mg | Reactivity | Human | Presentation | Aff - Purified | Applications | Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot | Isotype | Ig | Host | Rabbit | PDF datasheet | view | Shipping to | Worldwide | Synonyms | E2F-1, RBBP3, RBBP-3, PBR3, RBAP-1, RBAP1, FocusOn059 | Swiss Prot Num | Q01094 | Immunogen | This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S337 of human E2F1. | Product Type | Antibodies | Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. | References | 1. O'Donnell, K.A., et al., Nature 435(7043):839-843 (2005). 2. Wang, C., et al., J. Biol. Chem. 280(13):12339-12343 (2005). 3. Joshi, B., et al., Oncogene 24(13):2204-2217 (2005). 4. Saberwal, G., et al., Int. J. Hematol. 80(2):146-154 (2004). 5. Chaussepied, M., et al., Mol. Cell 16(5):831-837 (2004). | |
Format | State: Liquid purified Ig fraction. | ? | Purification: Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | ? | Buffer System: PBS with 0.09% (W/V) Sodium Azide as preservative. | |
Specificity | This antibody detects E2F1 pSer337. | Add. information | Molecular weight: 46920 Da | Application | ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. | Background | E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. | Caution | This product is for research use only. Not for use in diagnostic or therapeutic procedures. | Concentration | 0.25 mg/ml | Focuson | 059 Antibodies to E2F Transcription Factor Family | |
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