| Immunogen | Multi drug resistant chinese hamster ovary cell line, CHrC5 |
| Product Type | Antibodies |
| Recommended Isotype Control | SM10P (for use in human samples), AM03095PU-N |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Scheper, R.J. et al. (1988) Monoclonal antibody JSB-1 detects a highly conserved epitope on the P-glycoprotein associated with multi-drug resistance. Int. J. Cancer. 42: 389 - 394. 2. Bell, D.R. et al. (1985) Detection of P-glycoprotein in ovarian cancer. A molecular marker associated with multi drug resistance. J. Clin. Oncol. 3: 311 - 315. 3. Ma, D.D.F. et al. (1987) Detection of a multidrug resistant phenotype in acute non lymphoblastic leukaemia. Lancet. 1: 135. 4. Ling, V. (1992) Charles F. Kettering Prize. P-glycoprotein and resistance to anticancer drugs Cancer 69: 2603 - 2609 5. Fukuda et al. (1994) Characterization of newly established adriamycin resistant human leukemic cell lines (KYADR1 and KY-ADR2). Leuk. Res. 18: 709-715. |
|
| Format | State: Liquid Concentrated Tissue Culture Supernatant |
| ? | Buffer System: Phosphate buffered saline containing 0.09% Sodium Azide, 1% Bovine Serum Albumin |
|
| Specificity | This antibody recognises the 170-180kD P-glycoprotein, a plasma membrane associated molecule which is important in mediating resistance to cytotoxic drugs. In normal tissues P-glycoprotein is expressed in liver, kidney, colon, adrenal gland and lung. In malignant conditions increased expression of P-glycoprotein may be associated with multi-drug resistance, and the detection of this can have important consequences for treatment. This clone has also been reported to be suitable for Western blot analysis. |
| Application | Immunohistochemistry on frozen and paraffin* sections: 1/10 - 1/20; *This product requires protein digestion pre-treatment of paraffin sections e.g. 0.1% pronase, 10 mins. Histology Positive Control Tissue: Liver, kidney or lung. |
| Caution | (A full Health and Safety assessment is available upon request) This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
