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Cat. No |
Model |
Description |
Unit |
Price (VATº°µµ) |
³³±â |
Àç°í |
DataSheet |
ÁÖ¹®/°ßÀû |
LCA-23-352461 |
NBP1-05208 |
alpha Internexin Antibody//Rat/Chicken |
1/EA |
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¹®ÀÇ |
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Subject
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alpha Internexin Antibody |
Description
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/ Rat/Chicken |
Clonality
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Poly |
Company
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Novus biologicals |
Application
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IF, Western Blot |
Conjugation
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Immunogen
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Contents
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Description:Summary: Species: | Rt | Applications: | WB, IF | Clonality: | Polyclonal | | Gene: | INA | Purity: | IgG purified | Host: | Chicken | | Specificity: | alpha-Internexin | Publications: | Antibody has not yet been mentioned in a publication. | Details: Gene Symbol: INA | 24503 (Rat) | Related Genes: | ALB, C3, NR1I2, NPR1, CES2, CASP1, CFB, C4A, APOA1, CAT, NEFL, MGC42105, SEPSECS, PRPH, RAG1AP1, GAD1, CPM, LPA, UCN3, CD44, PRNP, CFH, GFAP, C5, NFASC, NEFM, PLG, CD3, RHO, HSPA8, MPO, KCNN4, SOD2, TNF, SNAP25, IKZF1, HNRNPC, GLYAT, C2, C1S | Background: Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin. a-internexin is an approx. 66 kDa Class IV intermediate filament originally discovered as it copurifies with other neurofilament subunits (1). It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed in large amounts early in neuronal development, but is down regulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. In addition recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration (2), and that alpha-internexin positive inclusions are the hallmark of a type of frontotemporal dementia known as neurofilament inclusion body disease (3). The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Species: Applications: Uses: | Immunofluorescence and Western blot On western blots use a brain or spinal cord homogenate and look for a major band at SDS-PAGE apparent molecular weight of 66 to 68kDa. The human protein runs at approx. 68kDa, while rat, mouse and other species run at approx. 66kDa. Minor bands at approx. 150kDa are probably covalent dimers, and bands at approx. 50kDa represent a-internexin breakdown products. | Dilutions: | immunofluorescence 1:1000, Western Blot 1:10000 | Packaging: Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. | Preservative: | No Preservative | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
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