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Subject
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F(ab)2 Fragment Antibody [HRP] |
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Description
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/ Human/Goat |
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Clonality
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Poly |
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Company
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Novus biologicals |
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Application
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ELISA, IHC-P, Western Blot |
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Conjugation
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Immunogen
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Contents
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Description:Summary: | Species: | Hu | | Applications: | WB, IHC, ELISA | | Clonality: | Polyclonal | | | Gene: | IGHG1 | | Purity: | Immunogen affinity purified | | Host: | Goat | | | Specificity: | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes. | | Publications: | Antibody has not yet been mentioned in a publication. | Details: | Conjugate: | HRP | | Isotype: | IgG | | Immunogen: | Human kappa chain | Gene Symbol: IGHG1 | Entrez: | 3500 (Human) | | Related Genes: | IGHG2, C3, Olfr48, C3, C3, Pgm1, Ada, Pgm2, Ada, RAG1, Hnrnpa2b1, Rag1, ADA, Pgm2, Rag1, Trf, Ier2, PGK1, A1BG, PGD, Actb, Darc, Blnk, IGLL1, Rag2, Gpi, SPANXB1, GYPE, PGM1, PPIA, BBS9, ABO, GYPA, Kel, GYPB, KEL, Hspb1, Cd79a, HSPB2, HSPB1 | Background: Human IgG consists of four subclasses (1-4) that can be recognized by antigen differences in their heavy chains. Each subclass has different biological and physiochemical properties. The IgG subclass may be preferentially produced in response to different antigens. The Fab fragment (fragment antigen binding) is the antigen region of the IgG molecule. It is composed of one constant and one variable domain of each of the heavy and the light chain. Species: Applications: | Uses: | Immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2?-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. | | Dilutions: | ELISA 1:200 to 1:1000, immunohistochemistry 1:200 to 1:1000, Western Blot 1:200 to 1:1000 | | Concentrations: | 1.0 mg/ml | Packaging: | Buffer: | Lyophilized 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA; pH 8.0 with 10 mg/ml Bovine S | | Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. | | Preservative: | 0.01% Gentamicin Sulfate | | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
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