| Immunogen | Synthetic peptide corresponding to 5-hydroxymethyluridine. |
| Product Type | Antibodies |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Cooney, R. et al. (2001) Association of tocopherols with circulating autoantibody levels against an oxidized DNA nucleoside in humans. Free. Radic. Biol. Med. 31:460 - 8. 2. Frenkel, K. et al. (1998) Serum autoantibodies recognizing 5hydroxymethyl2 doxyuridine, an oxidized DNA base, as biomarkers of cancer risk in women. Cancer. Epidemiol. Biomarkers. 4:49 - 57. |
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| Format | State: Liquid serum containing 0.09% Sodium Azide (NaN3) |
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| Specificity | This antibody recognises 5-hydroxymethyluridine, which is created by the oxidation of thymine by ionising radiation or reactive oxygen species. This oxidation causes changes in gene expression, mutations and chromosomal instability all of which play a role in cancer, autoimmune disease and aging. |
| Application | ELISA: 1:25000. |
| Background | 5-hydroxymethyluridine is a potential biomarker for female breast, colon and rectal cancers, being present at elevated levels, possibly due to impaired DNA repair mechanisms. |
| Caution | (A full Health and Safety assessment is available upon request) This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only. |
