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Description |
Unit |
Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-24-366939 |
AM05288PU-N |
6xHis-Tag// |
1/EA |
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문의 |
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Subject
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6xHis-Tag |
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Description
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Clonality
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Mono |
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Company
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Acris Antibodies GmbH |
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Application
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Western blot / Immunoblot |
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Conjugation
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Immunogen
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Contents
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Description: | Manufacturer | Acris Antibodies GmbH | | Quantity | 100 ug | | Presentation | Purified | | Applications | Western blot / Immunoblot | | Isotype | IgG2a | | Host | Mouse | | Clone | IPA2C5.1 | | PDF datasheet | view | | Shipping to | Worldwide | | Immunogen | Hybridoma produced by the fusion of splenocytes from mice immunized with His-His-His-His-His-His synthetic peptide and mouse myeloma cells. | | Product Type | Antibodies | | Storage | Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch. | | References | 1. Zentgraf, H., et al. "Detection of histidine-tagged fusion proteins by using a high-specific mouse monoclonal anti-histidine tag antibody." Nucleic Acids Res. 1995, 23, 3347-3348. 2. Sisk, W.P., et al. "High-level expression and purification of secreted forms of herpes simplex virus type 1 glycoprotein gD synthesized by baculovirus-infected insect cells." J. Virol. 1994, 68, 766-775. 3. Hosfield, T. and Lu, Q. "S. pombe expression vector with 6x(His) tag for protein purification and potential for ligation-independent cloning." Biotechniques 1999, 27, 50-60. | |
| Format | State: Liquid (sterile filtered) purified IgG fraction. | | ? | Purity: Purified | | ? | Buffer System: PBS containing 0.08% Sodium Azide as preservative. | |
| Specificity | This antibody is effective in detecting proteins expressed using the following vector systems, pET 28, 30 and 32 as well as Invitrogen's pIZ. Specific for 6x-His tags only and for His tags no longer than 6 histidines. | | Application | Western Blot. | | Background | 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems. | | Concentration | 1.0 mg/ml | |
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