| Synonyms | DOPA decarboxylase |
| Swiss Prot Num | P27718 |
| Immunogen | SDS denatured, recombinant bovine DOPA decarboxylase expressed E. coli and purified from inclusion bodies. |
| Product Type | Antibodies |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
|
| Format | State: Liquid purified IgG fraction. |
| ? | Purification: Affinity Chromatography. |
| ? | Buffer System: 10mM HEPES pH7.5, 150mM NaCl containing 0.09% Sodium Azide as preservative and 0.01% BSA, 50% Glycerol as stabilizer. |
|
| Specificity | This antibody recognises DOPA decarboxylase (aromatic-L-amino acid decarboxylase, DDC). |
| Application | Western Blot: 1/1000. This antibody detects a band of approximately 55kDa in rat adrenal medulla. |
| Background | DOPA decarboxylase is an enzyme that catalyses the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. The enzyme is expressed ubiquitously and is essential for the formation of catecholamines, indoleamines and trace amines. DDC is considered to be the rate-limiting step for the formation of trace amines, but not for the formation of catecholamines or indoleamines. It also becomes rate-limiting for dopamine formation in Parkinsons disease patients treated with L-DOPA. |
