| Synonyms | AP2-associated protein kinase 1, KIAA1048 |
| Swiss Prot Num | Q2M2I8 |
| Immunogen | This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human AAK1. |
| Product Type | Antibodies |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Henderson,D.M., Mol. Biol. Cell 18 (7), 2698-2706 (2007) 2. Takahashi,T., Cancer Res. 66 (24), 11932-11937 (2006) 3. Schmid,E.M., PLoS Biol. 4 (9), E262 (2006) |
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| Format | State: Liquid purified Ig |
| ? | Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS |
| ? | Buffer System: PBS with 0.09% (W/V) sodium azide |
|
| Specificity | This antibody reacts to AAK1. |
| Application | ELISA: 1/1,000. Western blotting: 1/50 - 1/100. |
| Background | Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. AAK1 is a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. |
| Caution | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Concentration | 0.25 mg/ml |
