| Synonyms | Alanine-tRNA ligase, Renal carcinoma antigen NY-REN-42 |
| Swiss Prot Num | P49588 |
| Immunogen | This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human AARS. |
| Product Type | Antibodies |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Shiba,K.,Biochemistry 34 (33), 10340-10349 (1995) 2. Ripmaster,T.L.,Proc. Natl. Acad. Sci. U.S.A. 92 (11), 4932-4936 (1995) 3. Sang Lee,J.,Cell 127 (3), 635-648 (2006) |
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| Format | State: Liquid purified Ig |
| ? | Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS |
| ? | Buffer System: PBS with 0.09% (W/V) sodium azide |
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| Specificity | This antibody reacts to AlaRS (AARS). |
| Application | ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50. |
| Background | The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases,of the class II enzymes.Class II tRNA synthases evolved early in evolution and are highly conserved.This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein.tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons.They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. |
| Caution | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Concentration | 0.25 mg/ml |
