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Description |
Unit |
Price
(VAT별도) |
납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-24-366995 |
AP12549PU-N |
ABL1//Human/Rabbit |
1/EA |
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문의 |
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Subject
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ABL1 |
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Description
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/ Human/Rabbit |
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Clonality
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Mono |
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Company
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Acris Antibodies GmbH |
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Application
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Enzyme Immunoassay, Paraffin Sections |
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Conjugation
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Immunogen
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Contents
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Description: | Manufacturer | Acris Antibodies GmbH | | Quantity | 0.1 mg | | Reactivity | Human | | Presentation | Aff - Purified | | Applications | Enzyme Immunoassay, Paraffin Sections | | Isotype | Ig | | Host | Rabbit | | PDF datasheet | view | | Shipping to | Worldwide | | Synonyms | c-ABL, p150, JTK7 | | Swiss Prot Num | P00519 | | Immunogen | This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S588 of human ABL. | | Product Type | Antibodies | | Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. | | References | 1. Saglio, G., et al., Cell. Mol. Life Sci. 61(23):2897-2911 (2004). 2. Deming, P.B., et al., Mol. Cell. Biol. 24(23):10289-10299 (2004). 3. Graux, C., et al., Nat. Genet. 36(10):1084-1089 (2004). 4. Monma, F., et al., Int. J. Hematol. 80(2):155-158 (2004). 5. Gustafson, W.C., et al., J. Biol. Chem. 279(10):9400-9408 (2004). | |
| Format | State: Liquid purified Ig fraction. | | ? | Purification: Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | | ? | Buffer System: PBS with 0.09% (W/V) Sodium Azide as preservative. | |
| Specificity | This antibody detects ABL1 pSer588. | | Add. information | Molecular weight: 124955 Da | | Application | ELISA: 1/1,000. Immunohistochemistry: 1/50-1/100. | | Background | The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11. Transcript Variant: Transcript variant b includes exon 1b, but not exon 1a, resulting in a different N-terminus. This variant contains an N-terminal glycine which could be myristylated and is thus postulated to be directed to the plasma membrane. | | Caution | This product is for research use only. Not for use in diagnostic or therapeutic procedures. | | Concentration | 0.25 mg/ml | |
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