| Synonyms | ATP-dependent DNA helicase VIII, HDH-VIII, G3BP |
| Swiss Prot Num | Q13283 |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human G3BP-1 around the phosphorylation site of serine 232 (S-S-SP-P-A). |
| Product Type | Antibodies |
| Storage | Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | 1. Tao WA, et al. (2005) Nat Methods. 2(8): 591-598. 2. Zheng H, et al. (2005) Mol Cell Proteomics. 4(6):721-730. 3. Beausoleil SA, et al. (2004) Proc Natl Acad Sci U S A.101 (33): 12130-12135. 4. Tourrihre H, et al. (2003) J Cell Biol.160 (6): 823-831. |
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| Format | State: Liquid purified Ig fraction. |
| ? | Purification: Immunoaffinity chromatography. |
| ? | Buffer System: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol. |
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| Specificity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. G3BP-1 (phospho-Ser232) antibody detects endogenous levels of G3BP-1 only when phosphorylated at serine 232. |
| Application | Suitable for use in Western blot (1:500~1:1000) and Immunohistochemistry (1:50~1:100). |
| Concentration | 1.0 mg/ml |
