Synonyms | GRB2-associated binding protein 1, GAB-1 |
Swiss Prot Num | Q13480 |
Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human Gab1 around the phosphorylation site of tyrosine 627 (V-E-YP-L-D). |
Product Type | Antibodies |
Storage | Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
References | 1. Anders Kallin, et al. (2004) J. Biol. Chem ; 279: 17897 - 17904. 2. Hideto Kameda, et al. (2001) Cell Growth Differ ; 12: 307. 3. Masaki Osawa, et al. (2002) J. Cell Biol ; 158: 773. |
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Format | State: Liquid purified IgG |
? | Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. |
? | Buffer System: PBS(without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol |
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Specificity | Gab1 antibody detects endogenous levels of Gab1 only when phosphorylated at tyrosine 627. |
Application | Western Blot: 1:500~1:1000. Immunohistochemistry: 1:50~1:100. |
Concentration | 1.0 mg/ml |