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Cat. No |
Model |
Description |
Unit |
Price (VATº°µµ) |
³³±â |
Àç°í |
DataSheet |
ÁÖ¹®/°ßÀû |
LCA-24-355858 |
AP02777PU-N |
GAB1//Human, Mouse/Rabbit |
1/EA |
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Subject
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GAB1 |
Description
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/ Human, Mouse/Rabbit |
Clonality
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Mono |
Company
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Acris Antibodies GmbH |
Application
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Western blot / Immunoblot |
Conjugation
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Immunogen
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Contents
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Description: Manufacturer | Acris Antibodies GmbH | Quantity | 100 ug | Reactivity | Human, Mouse | Presentation | Aff - Purified | Applications | Western blot / Immunoblot | Host | Rabbit | Shipping to | Worldwide | Synonyms | GRB2-associated binding protein 1, GAB-1 | Immunogen | The antiserum was produced against synthesized non-phosphopeptide derived from human Gab1 around the phosphorylation site of tyrosine 627 (V-E-YP-L-D). | Product Type | Antibodies | Storage | Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. | References | 1. Anders Kallin, et al. (2004) J. Biol. Chem; 279: 17897 - 17904. 2. Hideto Kameda, et al. (2001) Cell Growth Differ; 12: 307. 3. Masaki Osawa, et al. (2002) J. Cell Biol; 158: 773. | |
Format | State: Liquid purified IgG fraction. | ? | Purification: Affinity Chromatography using epitope-specific immunogen. | ? | Buffer System: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol. | |
Specificity | This antibody detects endogenous levels of total Gab1 protein. | Application | Western Blot: 1/500~1/1000. Immunohistochemistry: 1/50~1/100. | Background | Gab1 is a 115 kDa multiple docking protein that plays an essential role in cellular growth, transformation and apoptosis. Gab1 can be phosphorylated by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), platelet derived growth factor receptor beta] (PDGFRbeta]), hepatocyte growth factor/scatter factor receptor (HGFR/SFR or c Met), and epidermal growth factor receptor (EGF), as well as in response to cell cell adhesion. Gab1 is tyrosine phosphorylated on at least 16 sites, some of which serve as binding sites for phosphoatidylinositol 3 kinase (PI3K), Grb2, PLC gamma 1, Nck, and SHP2. Phosphorylation of Gab1 on tyrosines 627 and 659 is critical for its binding to SHP2, and for activation of the ERK/MAPK pathway in response to EGF. | Concentration | 1.0 mg/ml | |
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