Swiss Prot Num | P42771 |
Immunogen | Synthetic peptide from C-terminus of human p14ARF. |
Product Type | Antibodies |
Storage | Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch. |
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Format | State: Liquid purified Ig |
? | Buffer System: 10mM PBS, pH 7.4 with BSA and Sodium Azide |
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Specificity | This antibody reacts to p14ARF. |
Application | Western Blot: 1-2 µg/ml for 2hrs. Immunohistochemistry on paraffin sections: 1/25 for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Positive Control: Hela cells. Lymphoma |
Background | The INK4a-ARF locus encodes two unrelated proteins both of which function in tumor suppression. p14ARF arrests the cell cycle in a p53-dependent manner. p14ARF binds to mdm2 and promotes the rapid degradation of mdm2 proteinrequired for mdm2 modification and concurrent p53 stabilization and accumulation. This interaction is mediated by the exon 1Beta-encoded N-terminal domain of p14ARF and a C-terminal region of mdm2. Deletion of the ARF-INK4a locus simultaneously impairs both the INK4a-cyclin D/cdk4-RB and the ARF-mdm2-p53 pathways. |
Concentration | 0,2 mg/ml |