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Cat. No |
Model |
Description |
Unit |
Price (VATº°µµ) |
³³±â |
Àç°í |
DataSheet |
ÁÖ¹®/°ßÀû |
LCA-24-367174 |
AP01617PU-N |
I Kappa B alpha (IKBa)//Human, Mouse, Rat/Rabbit |
1/EA |
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Subject
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I Kappa B alpha (IKBa) |
Description
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/ Human, Mouse, Rat/Rabbit |
Clonality
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Mono |
Company
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Acris Antibodies GmbH |
Application
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Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot |
Conjugation
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Immunogen
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Contents
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Description: Manufacturer | Acris Antibodies GmbH | Quantity | 100 ug | Reactivity | Human, Mouse, Rat | Presentation | Aff - Purified | Applications | Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot | Host | Rabbit | PDF datasheet | view | Shipping to | Worldwide | Synonyms | NFKBIA, IKBA, MAD3, NFKBI, NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkappaBalpha, IkB-alpha | Swiss Prot Num | P25963 | Product Type | Antibodies | Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch. | |
Format | State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE). | ? | Purification: Affinity Chromatography using epitope-specific immunogen. | ? | Buffer System: Phosphate buffered saline (PBS), pH~7.2 containing 15 mM Sodium Azide as preservative. | |
Specificity | This antibody detects endogenous levels of IkB-alpha pSer32/36 protein. | Application | ELISA: 1/10000-1/20000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200. | Background | On the basis of both functional and structural considerations, members of the I?¨¬B family of proteins can be divided into four groups. The first of these groups, I?¨¬B-?¡¾ (a 35-37 kDa protein), includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NF?¨¬B complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to ?¨¬B sites, suggesting that the I?¨¬B-?¡¾ family binds to the p65 subunit of p50-p65 heterocomplex through Ankyrin repeats. The second member of the I?¨¬B family is represented by a 45 kDa protein designated I?¨¬B-?©÷. The third group of I?¨¬B proteins is represented by I?¨¬B-?©ø, a 70 kDa protein identical in sequence with the C-terminal domain of the p110 precursor of NF?¨¬B p50 and expressed predominantly in lymphoid cells. An additional I?¨¬B family member has been identified as I?¨¬B-u, a 45 kDa protein which has several phosphorylated forms and is primarily found complexed with RelA and/or c-Rel. | Concentration | 1.0 mg/ml | |
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