| Synonyms | Histone H3; H3 histone family; member K; H3 histone family, member I; H3 histone family, member L; HIST1H3A protein; HIST1H3F protein; HIST1H3E protein; HIST1H3B; HIST1H3A; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; |
| Immunogen | This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S28 of human H3. |
| Product Type | Antibodies |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch |
| References | 1. Lusic, M., et al., EMBO J. 22(24):6550-6561 (2003). 2. Deng, L., et al., Virology 289(2):312-326 (2001). 3. Deng, L., et al., Virology 277(2):278-295 (2000). 4. El Kharroubi, A., et al., Mol. Cell. Biol. 18(5):2535-2544 (1998). 5. Albig, W., et al., Hum. Genet. 101(3):284-294 (1997). |
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| Format | State: Liquid purified Ig fraction. |
| ? | Purification: Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| ? | Buffer System: PBS with 0.09% (W/V) Sodium Azide as preservative. |
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| Specificity | This antibody detects H3 pSer28. Predicted to cross react with Mouse and Zebrafish (100% Antigen Homology). |
| Add. information | Molecular weight: 15404 Da |
| Application | ELISA: 1/1,000. Western Blot: 1/100-1/500. |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. |
| Caution | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Concentration | 0.25 mg/ml |
