|
| |
| Cat. No |
Model |
Description |
Unit |
Price
(VAT별도) |
납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-23-351144 |
NBP1-19412 |
Aconitase 1 Antibody//Human, Mouse,/Rabbit |
1/EA |
|
|
|
|
문의 |
|
|
|
|
|
|
|
Subject
|
Aconitase 1 Antibody |
|
Description
|
/ Human, Mouse,/Rabbit |
|
Clonality
|
Poly |
|
Company
|
Novus biologicals |
|
Application
|
IF, IHC-P, Western Blot |
|
Conjugation
|
|
|
Immunogen
|
|
|
Contents
|
Description:Summary: | Species: | Rt, Mu, Hu | | Applications: | IF, IHC, WB | | Clonality: | Polyclonal | | | Gene: | ACO1 | | Purity: | Affinity purified | | Host: | Rabbit | | | Specificity: | IRP-1 (R134) detects endogenous levels of IRP-1 protein. | Details: Gene Symbol: ACO1 | Entrez: | 48 (Human) | | | 11428 (Mouse) | | | 50655 (Rat) | | Related Genes: | ACO2, IREB2, TFRC, WNT2, FH, ME3, ME1, ME2, CS, SOD2, CAT, TF, SOD1, EPR, FXN, INS, CASP3, SLC11A2, NOS2, PGD, DMRT1, NFS1, CHMP2B, CYCS, GAPDH, XDH, GSR, GPI, NANOS2, G6PD, GALT, PKC, SDHB, MDH2, HMOX1, ISYNA1, SFRP1, AK1, FRZB, FSTL1 | Background: The insulin receptor substrate-1 (IRS-1), a major substrate of the insulinreceptor, is phosphorylated in response to stimulation of cells by insulin,insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylatedon serine, threonine and tyrosine residues in a variety of tissues. Aninsulin-sensitive serine/threonine kinase casein kinase II mediates a portionof the insulin-stimulated serine/threonine phosphorylation of overexpressedIRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzedphosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylationsite catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylationof IRS-1 may be a component of the intracellular insulin signalingcascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosinebinding domain of IRS-1, which is responsible for the interaction withthe insulin receptor. The association of 14-3-3 with IRS-1 increases significantlyupon treatment with okadaic acid, a potent serine/ threonine phosphataseinhibitor. Therefore, the association of 14-3-3 protein may play arole in the regulation of insulin sensitivity by interrupting the associationbetween the insulin receptor and IRS-1. Species: | Reactivity: | Human, Mouse, Rat | Applications: | Uses: | This antibody is useful for Immunohistochemistry and Immunofluorescence.Immunohistochemistry analyzes of IRP-1 (R134) antibody in paraffin-embedded human colon carcinoma tissue. | | Dilutions: | Western Blot 1:500-1:1000,immunohistochemistry 1:50-1:200,immunofluorescence 1:50-1:200, | Packaging: | Buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2. | | Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. | | Preservative: | No Preservative | | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
|
|
|
|
