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Unit |
Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-23-351243 |
NBP1-20086 |
active Caspase 3 Antibody//Human, Mouse,/Rabbit |
1/EA |
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문의 |
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Subject
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active Caspase 3 Antibody |
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Description
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/ Human, Mouse,/Rabbit |
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Clonality
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Poly |
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Company
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Novus biologicals |
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Application
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IHC-P, Western Blot |
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Conjugation
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Immunogen
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Contents
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Description:Summary: | Species: | Rt, Mu, Hu | | Applications: | IHC, WB | | Clonality: | Polyclonal | | | Gene: | CASP3 | | Purity: | Affinity purified | | Host: | Rabbit | | | Specificity: | Actived-Caspase-3 p17 detects endogenous levels of Actived-Caspase-3 p17 protein. | Details: Gene Symbol: CASP3 | Entrez: | 836 (Human) | | | 12367 (Mouse) | | | 25402 (Rat) | | Related Genes: | CASP9, CASP8, BCL2, PARP1, CYCS, BAX, BCL2L1, FAS, FASLG, COL11A2, TP53, AKT1, CASP7, MAPK1, TNF, CASP1, CDKN1A, MAPK8, PIK3CG, XIAP, NFKB1, BID, TNFSF10, MAPK3, CASP6, EPHB2, D4S234E, TCEAL1, MAPK14, ANXA5, JUN, GRAP2, DIABLO, AHSA1, AIMP2, APAF1, CAT, PCNA, BAK1, CASP2 | Background: Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartatespecific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp 175-Ser 176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp 28-Ser 29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at a Asp 216-Gly 217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Species: | Reactivity: | Human, Mouse, Rat | Applications: | Uses: | This antibody is useful for Western Blot and Immunohistochemistry. Western blot analyzes of Actived-Caspase-3 p17 antibody in extracts from 293 cells with treatment of etoposide. Immunohistochemistry analyzes of Actived-Caspase-3 p17 antibody in paraffin-embedded human lung carcinoma tissue. | | Dilutions: | Western Blot 1:500-1:1000, immunohistochemistry 1:50-1:200, | Packaging: | Buffer: | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2. | | Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. | | Preservative: | No Preservative | | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
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